Briefly, fibroblasts were harvested and resuspended in DMEM at a concentration of 1 10 6 cells/ml
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However, TMAO insult alone was not sufficient to convert renal fibroblasts to myofibroblasts requiring synergy with a preexisting pathological state, i.e., pro-fibrotic signaling (second-hit) in the form of TGF-1 stimulation, the most prominent pro-fibrotic cytokine 46
Studies have revealed its potential to reduce markers of inflammation, such as C-reactive protein (CRP), which are associated with an increased risk of cardiovascular disease
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